猪AQP11基因微环表达载体的构建

doi:10.11843/j.issn.0366-6964.2014.01.002

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (1): 9-13.doi: 10.11843/j.issn.0366-6964.2014.01.002

猪AQP11基因微环表达载体的构建

郭准，高会贞，韩立强，郭豫杰，杨国宇^*

(河南农业大学农业部动物生化与营养重点开放实验室，郑州 450002)

收稿日期:2013-07-03 出版日期:2014-01-23 发布日期:2014-01-23
通讯作者: 杨国宇，教授，博士，主要从事动物生物化学研究，E-mail：haubiochem @163.com
作者简介:郭准（1987-），男，河南洛阳人，硕士生，主要从事动物生物化学研究，E-mail：13939064425@163.com
基金资助:
农业部“引进国际先进农业科学技术”（948）重点项目(2011-G35)

Construction of a Minicircle Expression Vector of Aquaporin11 Gene in Pig

GUO Zhun，GAO Hui-zhen，HAN Li-qiang，GUO Yu-jie，YANG Guo-yu^*

(Key Laboratory of Regulation on Animal Growth and Development of Ministry of Agriculture，Henan Agricultural University，Zhengzhou 450002，China)

Received:2013-07-03 Online:2014-01-23 Published:2014-01-23

摘要/Abstract

摘要：

旨在构建猪AQP11基因高效表达载体，观察该载体转染Hela细胞的转染效果。本研究通过PCR方法从猪肠道黏膜组织中克隆AQP11基因，构建了微环表达载体minicircle-AQP11，通过瞬时转染Hela细胞观察绿色荧光蛋白表达。结合测序结果表明，本研究成功地克隆了AQP11基因的全序列,包含了完整的CDS区，构建了AQP11基因的微环表达载体，在Hela细胞中观察到了持续1周的绿色荧光蛋白表达。这为进一步阐明AQP11合成的生化过程及功能调控提供新思路。

Abstract:

This study aimed to construct minicircle expression vector of AQP11 and observe the effect of transfection in Hela cells.AQP11 gene was amplified from the porcine intestinal mucosa and minicircle expression vector minicircle-AQP11 was constructed.The recombinant plasmid minicircle-AQP11 was transfected into Hela cells to investigate the expression of GFP by green fluorescent microscope.The sequencing results showed that the whole sequence of AQP11 gene containing a whole CDS was amplified successfully.Minicircle expression vector minicircle-AQP11 was constructed successfully.GFP was successfully expressed in Hela cells by the detection of bright green fluorescence for one week.Which provide new ideas for further elucidating the biochemical process and functional regulation of AQP11.

中图分类号:

郭准，高会贞，韩立强，郭豫杰，杨国宇. 猪AQP11基因微环表达载体的构建[J]. 畜牧兽医学报, 2014, 45(1): 9-13.

GUO Zhun，GAO Hui-zhen，HAN Li-qiang，GUO Yu-jie，YANG Guo-yu. Construction of a Minicircle Expression Vector of Aquaporin11 Gene in Pig[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 9-13.

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猪AQP11基因微环表达载体的构建

Construction of a Minicircle Expression Vector of Aquaporin11 Gene in Pig

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